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Mol Cell Biol. 2008 Apr;28(7):2426-36. doi: 10.1128/MCB.01874-07. Epub 2008 Jan 28.

TAZ promotes cell proliferation and epithelial-mesenchymal transition and is inhibited by the hippo pathway.

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  • 1Department of Biological Chemistry, School of Medicine, Molecular and Cellular Biology Laboratory, Institutes of Biomedical Sciences, Department of Biology, School of Life Science, Fudan University, Shanghai 200032, China. qlei@fudan.edu.cn

Abstract

TAZ is a WW domain containing a transcription coactivator that modulates mesenchymal differentiation and development of multiple organs. In this study, we show that TAZ is phosphorylated by the Lats tumor suppressor kinase, a key component of the Hippo pathway, whose alterations result in organ and tissue hypertrophy in Drosophila and contribute to tumorigenesis in humans. Lats phosphorylates TAZ on several serine residues in the conserved HXRXXS motif and creates 14-3-3 binding sites, leading to cytoplasmic retention and functional inactivation of TAZ. Ectopic expression of TAZ stimulates cell proliferation, reduces cell contact inhibition, and promotes epithelial-mesenchymal transition (EMT). Elimination of the Lats phosphorylation sites results in a constitutively active TAZ, enhancing the activity of TAZ in promoting cell proliferation and EMT. Our results elucidate a molecular mechanism for TAZ regulation and indicate a potential function of TAZ as an important target of the Hippo pathway in regulating cell proliferation tumorigenesis.

PMID:
18227151
[PubMed - indexed for MEDLINE]
PMCID:
PMC2268418
Free PMC Article

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