OBJECTIVE: To evaluate the efficacy of pericoital intravaginal applications of a mixture of Egyptian bee honey and royal jelly (H/RJ) in the midcycle for the treatment of infertility due to asthenozoospermia. METHODS: Sohag University Hospital and Asyut Gynecology and Infertility Clinic conducted a crossover study of 99 couples affected by asthenozoospermia. One group used midcycle pericoital vaginal applications of H/RJ and the other underwent a standard intrauterine insemination (IUI) procedure, for 3 cycles or until conception occurred for both groups. After a washout period of 2 months, the couples for whom no pregnancy occurred were crossed over. RESULTS: A total of 553 cycles were analyzed. There were 23 (8.1%) and 7 (2.6%) pregnancies per cycle, respectively, in the H/RJ and the IUI groups, and the difference was statistically significant (P<0.001). CONCLUSION: Using H/RJ intravaginally might be a simple and reasonably effective method of treating asthenozoospermia.