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1: J Chromatogr Sci. 2008 Jan;46(1):23-9.Click here to read Links

A sensitive high-throughput HPLC assay for simultaneous determination of everolimus and clobetasol propionate.

Kamberi M, Fu K, Lu J, Chemaly GM, Feder D.

Department of Analytical Sciences, Abbott Vascular, Inc., Santa Clara, CA 95054-2807, USA. kmarika55@hotmail.com

A novel sensitive high-throughput high-performance liquid chromatography assay is developed and validated for the simultaneous determination of everolimus and clobetasol propionate in pharmaceutical formulations. The chromatographic separation is achieved on a Zorbax Eclipse XDB-C18 reversed-phase column using a gradient elution, with solvent A: ammonium acetate (pH 6.8; 0.01 M) and solvent B: acetonitrile. The mean recovery ranges from 95.1% to 100.0% for clobetasol propionate and from 97.9% to 103.7% for everolimus. The limit of quantitation for each analyte is 0.02 microg/mL. The percent relative standard deviations are less than 3% for intra- and inter-day analyses. The proposed method can be used for the routine quality control of everolimus and clobetasol propionate in complex pharmaceutical formulations, especially the drug-delivery systems with a low total drug-load.

PMID: 18218184 [PubMed - indexed for MEDLINE]

Patient Drug Information

  • Sirolimus (Rapamune® )

    Sirolimus is used in combination with other medications to prevent rejection of kidney transplants. Sirolimus is in a class of medications called immunosuppressants. It works by suppressing the body's immune system.

  • Clobetasol (Clobevate® , Cormax® , Cormax® Scalp Application, ...)

    Clobetasol is used to treat the itching, redness, dryness, crusting, scaling, inflammation, and discomfort of various skin and scalp conditions.

  • Source: AHFS Consumer Medication Information