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J Phys Chem B. 2008 Feb 14;112(6):1576-9. doi: 10.1021/jp711527v. Epub 2008 Jan 24.

Label-free coherent anti-stokes Raman scattering imaging of coexisting lipid domains in single bilayers.

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  • 1Weldon School of Biomedical Engineering and Department of Chemistry, Purdue University, West Lafayette, IN 47907, USA.


Laser-scanning coherent anti-Stokes Raman scattering (CARS) microscopy was used to image lipid domains in single bilayers without any labeling. On the basis of the molecular packing density difference between liquid-disordered (Ld), liquid-ordered (Lo), and gel (So) phases, clear vibrational contrasts were generated between coexisting domains in a single bilayer of DOPC/DPPC (1:1) and DOPC/DPPC/cholesterol (4:4:2). The method reported here can be potentially applied to study phase segregation in live cell membranes which are highly heterogeneous and dynamic.

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