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Bioresour Technol. 2008 Sep;99(14):6156-61. doi: 10.1016/j.biortech.2007.12.027. Epub 2008 Jan 22.

Enhanced enzymatic hydrolysis of wheat straw by aqueous glycerol pretreatment.

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  • 1National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing 100080, China.


Considering the practical technology-economy of glycerol processing from oleochemicals industry, the ensuing work was proposed to further explore the atmospheric aqueous glycerol autocatalytic organosolv pretreatment (AAGAOP) to improve the enzymatic hydrolysis of lignocellulosic biomass. With the liquid-solid ratio of 20 g g(-1) at 220 degrees C for 3h, the AAGAOP enabled wheat straw to remove approximately 70% hemicelluloses and approximately 65% lignin, with approximately 98% cellulose retention. The pretreated fiber was achieved with approximately 90% of the enzymatic hydrolysis yield after 48 h. At oven-drying, dehydration was likely to cause the hornification of fiber, which was responsible for the low enzymatic hydrolysis of dried fiber. With SEM observations, the AAGAOP disrupted wheat straw into thin and fine fibrils, with a small average size and more surface area. The AAGAOP technique, as a novel strategy, enhanced the enzymatic hydrolysis of lignocellulosic biomass by removing the chemically compositional barrier and altering the physically structural impediment.

[PubMed - indexed for MEDLINE]
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