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Free Radic Biol Med. 2008 Mar 15;44(6):1023-31. doi: 10.1016/j.freeradbiomed.2007.11.021. Epub 2007 Dec 14.

TRP-2 specifically decreases WM35 cell sensitivity to oxidative stress.

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  • 1L'OREAL Recherche, 90 rue du Général Roguet, 92583 Clichy Cedex, France.


TRP-2 (dopachrome tautomerase) is a melanogenic enzyme whose expression was recently reported to modulate melanocyte response to different cytotoxic events. Here we studied a possible role of TRP-2 in the oxidative stress response in the amelanotic WM35 melanoma cell line. Cell viability assays showed that TRP-2 overexpression in WM35 cells reduced their sensitivity to oxidative stress. Comet assays linked TRP-2 expression to DNA damage protection, and high-performance liquid chromotography-tandem mass spectrometry experiments showed an increase in intracellular glutathione in TRP-2-overexpressing cells. These effects were specifically reversed when TRP-2 was silenced by RNA interference. Nevertheless, these properties appeared to depend on a particular cell environment because expression of TRP-2 failed to rescue HEK epithelial cells exposed to similar treatments.

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