Nat Methods. 2008 Feb;5(2):175-7. doi: 10.1038/nmeth.1178. Epub 2008 Jan 20.

Printing protein arrays from DNA arrays.

He M, Stoevesandt O, Palmer EA, Khan F, Ericsson O, Taussig MJ.

Source

Technology Research Group, The Babraham Institute, Cambridge CB22 3AT, UK.

Abstract

We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.

PMID:: 18204456; [PubMed - indexed for MEDLINE]

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Printing protein arrays from DNA arrays.
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