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World J Gastroenterol. 2008 Jan 28;14(4):511-7.

Chaga mushroom (Inonotus obliquus) induces G0/G1 arrest and apoptosis in human hepatoma HepG2 cells.

Author information

  • 1Vestibulocochlear Research Center, Wonkwang University School of Medicine, #344-2, Shinyoung-dong, Iksan, Jeonbuk 570-749, Korea.

Abstract

AIM:

To investigate the anti-proliferative and apoptotic effects of Chaga mushroom (Inonotus obliquus) water extract on human hepatoma cell lines, HepG2 and Hep3B cells.

METHODS:

The cytotoxicity of Chaga extract was screened by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay. Morphological observation, flow cytometry analysis, Western blot were employed to elucidate the cytotoxic mechanism of Chaga extract.

RESULTS:

HepG2 cells were more sensitive to Chaga extract than Hep3B cells, as demonstrated by markedly reduced cell viability. Chaga extract inhibited the cell growth in a dose-dependent manner, which was accompanied with G0/G1-phase arrest and apoptotic cell death. In addition, G0/G1 arrest in the cell cycle was closely associated with down-regulation of p53, pRb, p27, cyclins D1, D2, E, cyclin-dependent kinase (Cdk) 2, Cdk4, and Cdk6 expression.

CONCLUSION:

Chaga mushroom may provide a new therapeutic option, as a potential anticancer agent, in the treatment of hepatoma.

PMID:
18203281
[PubMed - indexed for MEDLINE]
PMCID:
PMC2681140
Free PMC Article

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