Format

Send to:

Choose Destination
See comment in PubMed Commons below
Biotechnol Prog. 2008 Jan-Feb;24(1):279-82. doi: 10.1021/bp070173t. Epub 2008 Jan 16.

A Study of the interaction of HEK-293 cells with streamline chelating adsorbent in expanded bed operation.

Author information

  • 1Biotechnology and pharmaceutical processes research unit (Bio-P2), Department of Chemical Engineering, Ecole Polytechnique, Montréal, Québec, Canada.

Abstract

Expanded bed adsorption (EBA) is an efficient protein purification process reducing time and steps of downstream processing (DSP) since nonclarified culture media can be processed directly without prior treatments such as filtration or centrifugation. However, cells and debris can interact with the adsorbent and affect bed stability as well as purification performance. To optimize EBA operating conditions these biomass/adsorbent interactions have to be understood and characterized. The adsorption of Human Embryonic Kidney cells (HEK 293) on unprimed and nickel-primed metal affinity adsorbent was studied in a closed loop EBA setup. With the unprimed adsorbent, the overall level of interaction observed was nonsignificant. With the nickel-primed adsorbent and an initial cell concentration ranging from 0.08 x 10(6) to 0.2 x 10(6) cells/mL, biomass/adsorbent interaction was found to be moderate and the adsorption apparent first-order kinetic rate constant was determined to be k = 0.009 to 0.011 min(-1).

PMID:
18197671
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley
    Loading ...
    Write to the Help Desk