Nat Methods. 2008 Feb;5(2):179-81. Epub 2008 Jan 13.

Pyrobayes: an improved base caller for SNP discovery in pyrosequences.

Quinlan AR, Stewart DA, Strömberg MP, Marth GT.

Source

Department of Biology, Boston College, 140 Commonwealth Avenue, Chestnut Hill, Massachusetts 02467, USA.

Abstract

Previously reported applications of the 454 Life Sciences pyrosequencing technology have relied on deep sequence coverage for accurate polymorphism discovery because of frequent insertion and deletion sequence errors. Here we report a new base calling program, Pyrobayes, for pyrosequencing reads. Pyrobayes permits accurate single-nucleotide polymorphism (SNP) calling in resequencing applications, even in shallow read coverage, primarily because it produces more confident base calls than the native base calling program.

PMID:: 18193056; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Grant Support

Publication Types

Research Support, N.I.H., Extramural

MeSH Terms

Grant Support

R01 HG003698/HG/NHGRI NIH HHS/United States

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