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Cell Immunol. 2007 Oct;249(2):101-7. doi: 10.1016/j.cellimm.2007.12.001. Epub 2008 Jan 11.

Differential interleukin-10 production stratified by -571 promoter polymorphism in purified human immune cells.

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  • 1Asthma and Allergic Diseases Center, Beirne Carter Center for Immunology Research, University of Virginia, 409 Lane Road, Room 5031, P.O. Box 801355, Charlottesville, VA 22908-1355, USA. js3ch@virginia.edu

Abstract

A C-to-A base substitution has been identified at bp -571 in the IL-10 promoter and has been linked to numerous diseases. To investigate the role of this polymorphism on IL-10 production, T cells, B cells and monocytes were enriched from peripheral blood from subjects either homozygous for the C or A allele. Treatment of monocytes and B cells with lipopolysaccharide from individuals homozygous for the C allele resulted in higher levels of IL-10 production as compared to monocytes from individuals homozygous for the A allele. Though not statistically significant, when B cells were treated with anti-IgM or T cells with concanavalin A higher levels of IL-10 were produced from individuals homozygous for the A allele. Changes in IL-10 protein production were paralleled by similar changes in IL-10 mRNA production. These results demonstrate that changes in IL-10 production observed due to the -571 genotype depend on both cell type and stimulus.

PMID:
18191118
[PubMed - indexed for MEDLINE]
PMCID:
PMC2274832
Free PMC Article
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