Format

Send to:

Choose Destination
See comment in PubMed Commons below
Biol Psychiatry. 2008 May 15;63(10):997-1000. doi: 10.1016/j.biopsych.2007.09.025. Epub 2008 Jan 11.

Modulation of D-serine levels in brains of mice lacking PICK1.

Author information

  • 1Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21287, USA.

Abstract

BACKGROUND:

D-serine is an endogenous coagonist of the N-methyl-D-aspartate subtype glutamate receptor. Genetic association studies have implicated genes coding for enzymes associated with D-serine metabolism in schizophrenia and bipolar disorder.

METHODS:

Protein expression of serine racemase (SR) and its binding partner, protein interacting with C-kinase (PICK1), were examined by Western blotting in brains from wildtype and PICK1 knockout mice. Levels of D-serine in wildtype and PICK1 mice were also examined by an established high-pressure liquid chromatography protocol.

RESULTS:

Expression of SR and PICK1 proteins was developmentally regulated. Although no change was observed in the level of SR protein, levels of D-serine were selectively decreased in the forebrain of neonatal PICK1 knockout mice, compared with those in wildtype mice.

CONCLUSIONS:

PICK1 may be involved in the regulation of brain D-serine levels and SR in a spatially and temporally specific manner.

PMID:
18191108
[PubMed - indexed for MEDLINE]
PMCID:
PMC2715963
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Write to the Help Desk