Chem Commun (Camb). 2008 Jan 28;(4):480-2. doi: 10.1039/b716115h. Epub 2007 Nov 13.

Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.

Heal WP, Wickramasinghe SR, Bowyer PW, Holder AA, Smith DF, Leatherbarrow RJ, Tate EW.

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Department of Chemistry, South Kensington Campus, Imperial College, London, UK.

Abstract

N-Myristoyl transferase-mediated modification with azide-bearing substrates is introduced as a highly selective and practical method for in vitro and in vivo N-terminal labelling of a recombinant protein using bioorthogonal ligation chemistry.

PMID:: 18188474; [PubMed - indexed for MEDLINE]

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In Vitro

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077503/Wellcome Trust/United Kingdom

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Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myri...
Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.
Chem Commun (Camb). 2008 Jan 28 ;(4):480-2. doi: 10.1039/b716115h. Epub 2007 Nov 13 .

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