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Nat Methods. 2008 Feb;5(2):163-5. doi: 10.1038/nmeth1170. Epub 2008 Jan 6.

Epitope tagging of endogenous proteins for genome-wide ChIP-chip studies.

Author information

  • 1Department of Genetics and Case Comprehensive Cancer Center, Case Western Reserve University, 10900 Euclid Avenue, Cleveland, Ohio 44106, USA.

Abstract

We developed a strategy to introduce epitope tag-encoding DNA into endogenous loci by homologous recombination-mediated 'knock-in'. The tagging method is straightforward, can be applied to many loci and several human somatic cell lines, and can facilitate many functional analyses including western blot, immunoprecipitation, immunofluorescence and chromatin immunoprecipitation-microarray (ChIP-chip). The knock-in approach provides a general solution for the study of proteins to which antibodies are substandard or not available.

PMID:
18176569
[PubMed - indexed for MEDLINE]
PMCID:
PMC2435063
Free PMC Article

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