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Nat Methods. 2008 Feb;5(2):159-61. doi: 10.1038/nmeth1171. Epub 2008 Jan 6.

Highly inclined thin illumination enables clear single-molecule imaging in cells.

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  • 1Biological Macromolecules Laboratory, National Institute of Genetics, Yata 1111, Mishima, Shizuoka 411-8540, Japan. mtoku@lab.nig.ac.jp

Erratum in

  • Nat Methods. 2008 May;5(5):455.

Abstract

We describe a simple illumination method of fluorescence microscopy for molecular imaging. Illumination by a highly inclined and thin beam increases image intensity and decreases background intensity, yielding a signal/background ratio about eightfold greater than that of epi-illumination. A high ratio yielded clear single-molecule images and three-dimensional images using cultured mammalian cells, enabling one to visualize and quantify molecular dynamics, interactions and kinetics in cells for molecular systems biology.

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PMID:
18176568
[PubMed - indexed for MEDLINE]
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