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J Am Chem Soc. 2008 Jan 23;130(3):784-5. doi: 10.1021/ja0759139. Epub 2008 Jan 1.

Selective fluorescent chemosensor for the bacterial alarmone (p)ppGpp.

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  • 1Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.

Abstract

We have developed the first selective fluorescent chemosensor (PyDPA) for (p)ppGpp, a bacterial and plant alarmone. By using pyrene-excimer fluorescence, PyDPA shows very good selectivity for (p)ppGpp from among other nucleotides in water. PyDPA was used for the real-time detection of in vitro ppGpp synthesis by bacterial ribosomal complexes.

PMID:
18166051
[PubMed - indexed for MEDLINE]
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