Immunol Invest. 2007;36(5-6):649-64.

Dye dilution proliferation assay: application of the DDPA to identify tumor-specific T cell precursor frequencies in clinical trials.

Schwaab T, Fisher JL, Meehan KR, Fadul CE, Givan AL, Ernstoff MS.

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Medical Oncology Immunotherapy Program, Dartmouth Medical School, Lebanon, New Hampshire, USA.

Abstract

A better understanding of immune effector and regulatory pathways has led to innovative, and complex, immunotherapy strategies. CD8(+) cytolytic T lymphocytes (CTL) provide one common pathway of tumor cell destruction. The peripheral blood CTL compartment typically comprises a minority of anti-tumor CD8(+) lymphocytes and the determination of their number during clinical trials is the focus of various laboratory methods. We have monitored tumor specific CD8(+) as well as CD4(+) lymphocyte precursor frequencies in the peripheral blood using a Dye Dilution Proliferation Assay (DDPA). We summarize our experience applying DDPA in a multi-parameter, antigen-specific assay, detailing some of its complexities and advantages. We provide examples of our clinical trial results showing tumor-specific CD8(+) and CD4(+) precursor frequency (PF) data in patients being treated on novel immunotherapy trials.

PMID:: 18161523; [PubMed - indexed for MEDLINE]

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R01 CA095648/CA/NCI NIH HHS/United States

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