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1: J Am Chem Soc. 2008 Jan 23;130(3):794-5. Epub 2007 Dec 23.Click here to read Links

Paramagnetic relaxation-based 19f MRI probe to detect protease activity.

Mizukami S, Takikawa R, Sugihara F, Hori Y, Tochio H, Wälchli M, Shirakawa M, Kikuchi K.

Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, Osaka 565-0871, Japan.

A novel design principle for 19F MRI probes detecting protease activity was developed. This principle is based on 19F MRI signal quenching by the intramolecular paramagnetic effect from Gd3+. The intramolecular Gd3+ dramatically attenuated the 19F probe signal, and the paramagnetic effect was cancelled by the probe hydrolyzation by caspase-3. Using this probe, it was shown that the probe could detect caspase-3 activity spatially from a phantom image using 19F MRI.

PMID: 18154336 [PubMed - indexed for MEDLINE]