Determination of cytosolic ADP and AMP concentrations and the free energy of ATP hydrolysis in human muscle and brain tissues with 31P NMR spectroscopy

Magn Reson Med. 1991 Dec;22(2):505-11. doi: 10.1002/mrm.1910220258.

Abstract

Based on experimentally determined concentrations of PCr, Pi, ATP, and H+, the ADP and AMP concentrations and the free energy for the ATP hydrolysis in normal human brain and muscle were calculated assuming that the creatine kinase- and the myokinase-catalyzed reactions were in equilibrium, accounting for changes in pH and free Mg2+ ion concentration.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenosine Diphosphate / analysis*
  • Adenosine Monophosphate / analysis*
  • Adenosine Triphosphate / metabolism*
  • Brain / metabolism*
  • Cytosol / metabolism
  • Energy Metabolism
  • Humans
  • Hydrolysis
  • Magnetic Resonance Spectroscopy*
  • Muscles / metabolism*

Substances

  • Adenosine Monophosphate
  • Adenosine Diphosphate
  • Adenosine Triphosphate