Family 1 LTP sequence and structure. (A) Cutaway image showing wheat LTP2 bound to L-α-palmitoyl-phosphatidyl glycerol (redrawn from the coordinates of PDB 1N89) (Pons et al. 2003). (B) Stereoview of the same model highlighting the four helix structure of family 2 LTPs with disulfide bridges in red. Images were constructed as described for Figure 1. (C) Sequence logo showing the conserved features of family 2 LTPs from Arabidopsis thaliana, rice, and poplar. Disulfide pairs are indicated above the logo. Residue numbers and helices according to the wheat LTP2 structure are shown below. Logo was prepared as for Figure 1. The sequences considered were translated from the following gene models: A. thaliana At1g66850, At5g38160, At1g48750, At5g38195, At3g18280, At5g38170, At1g43667, At2g14846, At3g57310, At1g73780, At5g38180, At1g43666, At4g12825, At1g07747, At3g29152; rice Os03g0111300, Os10g0505700, Os10g0504900, Os05g0550300, Os10g0505500, Os10g0505000, Os05g0550600, Os06g0705400, Os11g0620300, Os01g0691100, Os01g0691300; poplar (JGI assembly v 1.1) grail3.0021013901, estExt_Genewise1_v1.C_LG_IV3319, estExt_Genewise1_v1.C_LG_XV0799, gw1.XII.565.1.

Family 1 LTP sequence and structure. (A) Cutaway view showing the lipid binding pocket of rice LTP1 occupied by myristic acid (redrawn from the coordinates of PDB 1UVA) (Cheng et al. 2004b). (B) Stereoview of the same model highlighting the four helix structure of family 1 LTPs with disulfide bridges in red. Both structures were rendered using UCSF Chimera (Pettersen et al. 2004) with surfaces calculated by the MSMS package (Sanner et al. 1996); (C) Sequence logo showing the conserved features of family 1 LTPs from Arabidopsis thaliana, rice, and poplar. Disulfide pairs are indicated above the logo. Residue numbers and helices according to the rice LTP1 structure are shown below the sequence. Mature peptides were predicted using SignalP 3.0 (Bendtsen et al. 2004) and aligned using ClustalW (Chenna et al. 2003). Logo was calculated using WebLogo (Crooks et al. 2004) after manually removing nonconserved gaps in the alignment. The sequences considered were translated from the following gene models: A. thaliana At2g15050, At2g15325, At2g18370, At2g38530, At2g38540, At3g08770, At3g51590, At3g51600, At4g33355, At5g01870, At5g59310, At5g59320; rice Os01g0219500, Os01g0822900, Os03g0808500, Os08g0131200, Os11g0114900, Os11g0115100, Os11g0115400, Os11g0427800, Os12g0114500, Os12g0115000, Os12g0115100, Os12g0115300, Os12g0115500; poplar (JGI assembly v 1.1) estExt_fgenesh4_pg.C_65530001, estExt_Genewise1_v1.C_LG_IV3602, estExt_Genewise1_v1.C_LG_VI1197, estExt_Genewise1_v1.C_LG_XVI0292, fgenesh4_pg.C_LG_XI000197, fgenesh4_pg.C_LG_XI000199, fgenesh4_pg.C_LG_XII001150, fgenesh4_pm.C_LG_IX000642, fgenesh4_pm.C_scaffold_148000018, grail3.0035029301, grail3.0038010001, grail3.0076005601, grail3.0076005701, grail3.0148004401, gw1.1437.5.1, gw1.40.321.1, gw1.I.5890.1, gw1.XVI.307.1.