Department of Radiology and Radiological Sciences, Vanderbilt University, 1161 21st Avenue South, Nashville, Tennessee 37232, USA. tk.sinha@vanderbilt.edu
We have developed a method for integrating three dimensional-volume reconstructions of spatially resolved matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) ion images of whole mouse heads with high-resolution images from other modalities in an animal-specific manner. This approach enabled us to analyze proteomic profiles from MALDI IMS data with corresponding in vivo data provided by magnetic resonance imaging.