FEBS Lett. 2008 Jan 23;582(2):229-32. Epub 2007 Dec 17.

TRPA1 channel activation induces cholecystokinin release via extracellular calcium.

Purhonen AK, Louhivuori LM, Kiehne K, Kerman KE, Herzig KH.

Source

Department of Biotechnology and Molecular Medicine, A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, Kuopio, Finland.

Abstract

TRPA1 channels are non-selective cation channels activated by plant derived pungent products including allyl isothiocyanate (AITC) from mustard. Therefore, possible intestinal secretory functions of these channels were investigated. We detected TRPA1 mRNA in mouse and human duodenal mucosa and in intestinal mouse neuroendocrine STC-1 cells. Stimulation of STC-1 cells with AITC increased intracellular calcium ([Ca(2+)](i)) and significantly stimulated cholecystokinin secretion by 6.7-fold. AITC induced cholecystokinin release was completely blocked by TRPA1 antagonist ruthenium red and depletion of extracellular calcium and reduced by 36% by nimodipine and nifedipine. This suggests that spices in our daily food might stimulate digestive functions.

PMID:: 18082143; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Molecular Biology Databases

CALCIUM, ELEMENTAL - HSDB

Supplemental Content

Save items

Related citations in PubMed

Activation of transient receptor potential A1 channels by mustard oil, tetrahydrocannabinol and Ca2+ reveals different functional channel states. [Neuroscience. 2008]
Activation of transient receptor potential A1 channels by mustard oil, tetrahydrocannabinol and Ca2+ reveals different functional channel states.
Cavanaugh EJ, Simkin D, Kim D. Neuroscience. 2008 Jul 17; 154(4):1467-76. Epub 2008 May 2.
Differentiation dependent expression of TRPA1 and TRPM8 channels in IMR-32 human neuroblastoma cells. [J Cell Physiol. 2009]
Differentiation dependent expression of TRPA1 and TRPM8 channels in IMR-32 human neuroblastoma cells.
Louhivuori LM, Bart G, Larsson KP, Louhivuori V, Näsman J, Nordström T, Koivisto AP, Akerman KE. J Cell Physiol. 2009 Oct; 221(1):67-74.
Molecular cloning and characterization of dog TRPA1 and AITC stimulate the gastrointestinal motility through TRPA1 in conscious dogs. [Eur J Pharmacol. 2009]
Molecular cloning and characterization of dog TRPA1 and AITC stimulate the gastrointestinal motility through TRPA1 in conscious dogs.
Doihara H, Nozawa K, Kawabata-Shoda E, Kojima R, Yokoyama T, Ito H. Eur J Pharmacol. 2009 Sep 1; 617(1-3):124-9. Epub 2009 Jul 1.
Overexpression of human transient receptor potential M5 upregulates endogenous human transient receptor potential A1 in a stable HEK cell line. [Assay Drug Dev Technol. 2010]
Overexpression of human transient receptor potential M5 upregulates endogenous human transient receptor potential A1 in a stable HEK cell line.
Buber MT, Cerne R, Cortés RY, Bryant RW, Lee SP. Assay Drug Dev Technol. 2010 Dec; 8(6):695-702. Epub 2010 Dec 6.
Review Properties and therapeutic potential of transient receptor potential channels with putative roles in adversity: focus on TRPC5, TRPM2 and TRPA1. [Curr Drug Targets. 2011]
Review Properties and therapeutic potential of transient receptor potential channels with putative roles in adversity: focus on TRPC5, TRPM2 and TRPA1.
Jiang LH, Gamper N, Beech DJ. Curr Drug Targets. 2011 May; 12(5):724-36.

See reviews... See all...

Cited by 8 PubMed Central articles

TRPA1 is a polyunsaturated fatty acid sensor in mammals. [PLoS One. 2012]
TRPA1 is a polyunsaturated fatty acid sensor in mammals.
Motter AL, Ahern GP. PLoS One. 2012; 7(6):e38439. Epub 2012 Jun 19.
Modulation of mouse gastrointestinal motility by allyl isothiocyanate, a constituent of cruciferous vegetables (Brassicaceae): evidence for TRPA1-independent effects. [Br J Pharmacol. 2012]
Modulation of mouse gastrointestinal motility by allyl isothiocyanate, a constituent of cruciferous vegetables (Brassicaceae): evidence for TRPA1-independent effects.
Capasso R, Aviello G, Romano B, Borrelli F, De Petrocellis L, Di Marzo V, Izzo AA. Br J Pharmacol. 2012 Mar; 165(6):1966-77.
TRPA1 contributes to specific mechanically activated currents and sensory neuron mechanical hypersensitivity. [J Physiol. 2011]
TRPA1 contributes to specific mechanically activated currents and sensory neuron mechanical hypersensitivity.
Brierley SM, Castro J, Harrington AM, Hughes PA, Page AJ, Rychkov GY, Blackshaw LA. J Physiol. 2011 Jul 15; 589(Pt 14):3575-93. Epub 2011 May 9.

See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
MedGen
Related information in MedGen
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

TRPA1 channel activation induces cholecystokinin release via extracellular calci...
TRPA1 channel activation induces cholecystokinin release via extracellular calcium.
FEBS Lett. 2008 Jan 23 ;582(2):229-32. Epub 2007 Dec 17 .

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: