Methods Mol Biol. 2007;368:313-24.

Cryopreservation of mammalian oocytes.

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Department of Obstetrics and Gynaecology, Wales College of Medicine, Cardiff University, UK.

Abstract

Two methods for the cryopreservation of mammalian oocytes are described. One method uses a relatively low concentration of the cryoprotectant propanediol plus sucrose and requires controlled-rate cooling equipment to achieve a slow cooling rate. Such a method has produced live births from cryopreserved human oocytes. The second method described employs a high concentration of the cryoprotectant dimethyl sulfoxide plus a low concentration of polyethylene glycol. This method involves cooling by plunging standard straws into liquid nitrogen vapor, hence avoiding the need for specialized equipment, but requires technical ability to manipulate the oocytes quickly in the highly concentrated cryoprotectant solutions. Murine oocytes vitrified, using this technique, have resulted in live births.

PMID:: 18080480; [PubMed - indexed for MEDLINE]

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Review Cryopreservation of mammalian embryos. [Methods Mol Biol. 2007]
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Cited by 2 PubMed Central articles

Effect of cryopreservation on acetylation patterns of lysine 12 of histone H4 (acH4K12) in mouse oocytes and zygotes. [J Assist Reprod Genet. 2010]
Effect of cryopreservation on acetylation patterns of lysine 12 of histone H4 (acH4K12) in mouse oocytes and zygotes.
Suo L, Meng Q, Pei Y, Fu X, Wang Y, Bunch TD, Zhu S. J Assist Reprod Genet. 2010 Dec; 27(12):735-41. Epub 2010 Sep 14.
The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. I: methodology. [BMC Cell Biol. 2010]
The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. I: methodology.
Deutsch M, Afrimzon E, Namer Y, Shafran Y, Sobolev M, Zurgil N, Deutsch A, Howitz S, Greuner M, Thaele M, et al. BMC Cell Biol. 2010 Jul 7; 11:54. Epub 2010 Jul 7.

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