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Genome Biol. 2007;8(12):R266.

Direct selection and phage display of a Gram-positive secretome.

Author information

  • 1Institute of Molecular Biosciences, Massey University, Palmerston North, New Zealand. d.jankovic@massey.ac.nz

Abstract

Surface, secreted and transmembrane protein-encoding open reading frames, collectively the secretome, can be identified in bacterial genome sequences using bioinformatics. However, functional analysis of translated secretomes is possible only if many secretome proteins are expressed and purified individually. We have now developed and applied a phage display system for direct selection, identification, expression and purification of bacterial secretome proteins.

PMID:
18078523
[PubMed - indexed for MEDLINE]
PMCID:
PMC2246268
Free PMC Article

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