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Genome Biol. 2007;8(12):R265.

Quantification of global transcription patterns in prokaryotes using spotted microarrays.

Author information

  • 1Department of Pathology and Infectious Diseases, Royal Veterinary College, Royal College Street, London, NW1 0TU, UK.

Abstract

We describe an analysis, applicable to any spotted microarray dataset produced using genomic DNA as a reference, that quantifies prokaryotic levels of mRNA on a genome-wide scale. Applying this to Mycobacterium tuberculosis, we validate the technique, show a correlation between level of expression and biological importance, define the complement of invariant genes and analyze absolute levels of expression by functional class to develop ways of understanding an organism's biology without comparison to another growth condition.

PMID:
18078514
[PubMed - indexed for MEDLINE]
PMCID:
PMC2246267
Free PMC Article

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