Format

Send to:

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2008 Jan;5(1):41-3. Epub 2007 Dec 9.

Bottom-up genome assembly using the Bacillus subtilis genome vector.

Author information

  • 1Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan. mita2001@sfc.keio.ac.jp

Abstract

We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.

Comment in

PMID:
18066072
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group
    Loading ...
    Write to the Help Desk