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1: Nat Methods. 2008 Jan;5(1):41-3. Epub 2007 Dec 9.Click here to read Links
Comment in:
Nat Methods. 2008 Jan;5(1):27-8.

Bottom-up genome assembly using the Bacillus subtilis genome vector.

Itaya M, Fujita K, Kuroki A, Tsuge K.

Mitsubishi Kagaku Institute of Life Sciences, 11 Minamiooya, Machida-shi, Tokyo 194-8511, Japan. mita2001@sfc.keio.ac.jp

We established a protocol to construct complete recombinant genomes from their small contiguous DNA pieces and obtained the genomes of mouse mitochondrion and rice chloroplast using a B. subtilis genome (BGM) vector. This method allows the design of any recombinant genomes, valuable not only for fundamental research in systems biology and synthetic biology but also for various applications in the life sciences.

PMID: 18066072 [PubMed - indexed for MEDLINE]

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