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Nat Methods. 2008 Jan;5(1):45-7. Epub 2007 Dec 9.

In vivo imaging of Drosophila melanogaster pupae with mesoscopic fluorescence tomography.

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  • 1Center for Molecular Imaging Research, Massachusetts General Hospital and Harvard Medical School, Building 149, 13th Street, Charlestown, Massachusetts 02129, USA. cvinegoni@mgh.harvard.edu

Abstract

We report a technique for fluorescence tomography that operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The method uses multi-projection illumination and photon transport description in opaque tissues. We demonstrate whole-body three-dimensional visualization of the morphogenesis of GFP-expressing salivary glands and wing imaginal discs in living Drosophila melanogaster pupae in vivo and over time.

PMID:
18066071
[PubMed - indexed for MEDLINE]
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