Database schema for PlantGDB, showing data sources, update frequency, computation and web services. PlantGDB is accessible at http://www.plantgdb.org, and genome browsers are accessible at http://www.plantgdb.org/XxGDB, where Xx is the first letter of the genus and species (e.g. AtGDB = Arabidopsis thaliana genome database).

Transcript assemblies (PUTs) at PlantGDB, grouped by taxonomic affiliation. Sequence totals displayed here are as of 30 October 2007. Parentheses indicate the number of species/subspecies per genus. Genera highlighted in yellow are associated with a genome browser at PlantGDB; an underscore indicates chromosome-based genome browsers. An asterisk designates genera for which PlantGDB provides preprocessed GeneSeqer indices for quick access to spliced alignments.

Screenshots from ZmGDB and yrGATE illustrate the use of online tools for gene discovery and community gene annotation. (A) A web-accessible table of Z. mays BACs (alternately shaded) displaying (left to right) the BAC GI, BAC clone name, followed by the ID, start/end coordinates and functional annotation of splice-aligned TIGR-predicted proteins from O. sativa and finally the ZmGDB entry date. All fields are searchable and each row is linked via column 1 to a genome browser view of the BAC region. This table is currently updated daily at ZmGDB (http://www.plantgdb.org/ZmGDB/DisplayGeneAnn.php). (Similar tables are available for eight other BAC-based xGDB browsers.) Note that a region of BAC GI 156523432 is aligned to three paralogous rice predicted polypeptides, annotated as ‘autophagy-related protein 8 precursor’. Clicking on the BAC GI ‘156523432’ in table column 1 (circled) brings up a BAC/Clone Context View of the specified region (B), showing spliced alignments to the rice predicted polypeptides (black), along with other alignment data, in this case maize cDNAs (blue) and maize ESTs (red). Note the evidence for alternative splicing among the maize ESTs (circles) suggesting at least two alternate transcripts (labeled 1 and 2). The user has the option to explore and annotate this variation using yrGATE. (C) Launching the yrGATE annotation tool displays scrolling list of evidence scores and supporting exons for all exon coordinates at a locus (alternative splice coordinates for 1 and 2 are circled). The user can build a complete gene model on screen by selecting each desired exon and then compare the resulting open reading frame to known proteins using BLAST (data not shown). (D) The chosen gene model is displayed graphically and will be published on the ZmGDB browser following curation by PlantGDB staff. Shown here are yrGATE models for the two putative splice variants, with translation start/stop positions indicated by triangles. (E) Predicted protein sequence for the two yrGATE gene models. This example illustrates how xGDB and yrGATE can be used to identify and publish gene model predictions quickly and easily, enhancing the community genome knowledge base for maize as well as facilitating hypothesis-driven research.