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Curr Microbiol. 2008 Apr;56(4):376-81. Epub 2007 Dec 6.

Optimization of sterilization of Escherichia coli in milk by surfactin and fengycin using a response surface method.

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  • 1College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, People's Republic of China.

Abstract

In this paper, the sensitivity of Escherichia coli to surfactin and fengycin was observed, and the optimization of the antimicrobial activity of surfactin and fengycin to E. coli in milk by a response surface methodology was studied. Results showed that E. coli had high sensitivity to these antibiotics, whose minimal inhibitory concentrations were 15.625 microg.mL(-1) and 31.25 microg.mL(-1), respectively. The optimization result indicated that E. coli could be sterilized by 5 orders of magnitude when the temperature was 5.5 degrees C, the action time was 15.8 h, and the concentration (surfactin/fengycin weight ratio 1:1) was 14.63 microg.mL(-1).

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