Send to:

Choose Destination
See comment in PubMed Commons below
J Agric Food Chem. 2007 Dec 26;55(26):10599-604. Epub 2007 Dec 4.

Cholesterol lowering mechanism of soybean protein hydrolysate.

Author information

  • 1Graduate School of Biotechnology, Korea University, Seoul, Korea.


Numerous attempts have been made to find the mechanism and component of the cholesterol lowering activity of soybean. In this study, it was proved that the peptides in soybean protein hydrolysate (SPH) made by certain proteases have a hypocholesterolemic effect. Among the mechanisms suggested, that is, blockage of bile acid and/or cholesterol absorption, inhibition of cholesterol synthesis, and stimulation of low-density lipoprotein receptor (LDL-R) transcription, SPH appeared to stimulate LDL-R transcription. When Hep T9A4 cells were incubated with soy protein hydrolysates by using the proteases from Bacillus amyloliquefaciens FSE-68, LDL-R transcription was strongly stimulated, but the other mechanisms were not affected. Among the six types of SPH, F1-15, hydrolyzed with the neutral protease to a degree of hydrolysis (DH) of 15%, showed the highest LDL-R transcription. The fractions of molecular weight of 200 and 3000 Da showed LDL-R transcription stimulating activity. The bioactivity is due to soybean peptides because the ethanol extract of soybean protein which contains isoflavones does not stimulate LDL-R transcription. In conclusion, dietary upregulation of LDL-R transcription by soybean may be consequent to an enhanced catabolism or a reduced synthesis of intracellular cholesterol. Therefore, we suggest that soy peptides can effectively stimulate LDL-R transcription in the human liver cell line and reduce blood cholesterol level.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Write to the Help Desk