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Genome Biol. 2007;8 Suppl 1:S7.

Tol2: a versatile gene transfer vector in vertebrates.

Author information

  • Division of Molecular and Developmental Biology, National Institute of Genetics, and Department of Genetics, The Graduate University of Advanced Studies (SOKENDAI), Mishima, Shizuoka 411-8540, Japan. kokawaka@lab.nig.ac.jp

Abstract

The medaka fish Tol2 element is an autonomous transposon that encodes a fully functional transposase. The transposase protein can catalyze transposition of a transposon construct that has 200 and 150 base pairs of DNA from the left and right ends of the Tol2 sequence, respectively. These sequences contain essential terminal inverted repeats and subterminal sequences. DNA inserts of fairly large sizes (as large as 11 kilobases) can be cloned between these sequences without reducing transpositional activity. The Tol2 transposon system has been shown to be active in all vertebrate cells tested thus far, including zebrafish, Xenopus, chicken, mouse, and human. In this review I describe and discuss how the Tol2 transposon is being applied to transgenic studies in these vertebrates, and possible future applications.

PMID:
18047699
[PubMed - indexed for MEDLINE]
PMCID:
PMC2106836
Free PMC Article

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