Studies on the expression of heat shock proteins during development in Drosophila clearly show that individual Hsps accumulate in a tissue- and developmental stage-specific manner. This is in contrast to their coordinate expression in response to stress. Therefore, the Hsps may play at least two roles, one as housekeeping proteins during development and/or differentiation and the second one in restoring cellular functions after environmental stress. Research in the first two decades following the discovery of the heat shock response have focused on a search for functions in stressed cells. The next few years should bring us further understanding on the role of these fascinating proteins during development in Drosophila as well as in other eukaryotes.