Nucleic Acids Symp Ser (Oxf). 2007;(51):377-8.

Molecular switch in tandem winged-helix motifs of elongation factor SelB.

Source

Laboratoire de Chimie et Biologie Structurales, ICSN-CNRS, 1 ave de la terrasse, 91190 Gif sur Yvette, France.

Abstract

Elongation factor SelB is responsible for cotranslational incorporation of the 21(st) amino acid selenocysteine (Sec) into proteins. UGA stop codon is recoded as a Sec codon in presence of a downstream mRNA hairpin. Prokaryotic SelB has EF-Tu-like N-terminal domains and a C-terminal extension containing four tandem winged-helix motifs (WH1-4). The C-terminal extension recognizes the mRNA hairpin. Crystal structures of the Escherichia coli WH3/4 domains and WH1-4 domains from Moorella thermoacetica each bound to mRNA were determined. These structures provide insights into how the molecular switch that may allow communication between tRNA and mRNA binding sites is formed and illustrate how RNA acts as an activator of the switch. The structures show that tandem WH motifs not only provide an excellent scaffold for RNA binding but can also have an active role in the function of protein-RNA complexes.

PMID:: 18029744; [PubMed - indexed for MEDLINE]

Free full text

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Supplemental Content

Save items

Related citations in PubMed

Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB. [J Mol Biol. 2007]
Structural insight into a molecular switch in tandem winged-helix motifs from elongation factor SelB.
Soler N, Fourmy D, Yoshizawa S. J Mol Biol. 2007 Jul 20; 370(4):728-41. Epub 2007 May 10.
Crystal structure of an mRNA-binding fragment of Moorella thermoacetica elongation factor SelB. [EMBO J. 2002]
Crystal structure of an mRNA-binding fragment of Moorella thermoacetica elongation factor SelB.
Selmer M, Su XD. EMBO J. 2002 Aug 1; 21(15):4145-53.
Recognition of the mRNA selenocysteine insertion sequence by the specialized translational elongation factor SELB. [Genes Dev. 1994]
Recognition of the mRNA selenocysteine insertion sequence by the specialized translational elongation factor SELB.
Ringquist S, Schneider D, Gibson T, Baron C, Böck A, Gold L. Genes Dev. 1994 Feb 1; 8(3):376-85.
Review Protein factors mediating selenoprotein synthesis. [Curr Protein Pept Sci. 2002]
Review Protein factors mediating selenoprotein synthesis.
Lescure A, Fagegaltier D, Carbon P, Krol A. Curr Protein Pept Sci. 2002 Feb; 3(1):143-51.
Review An extended Escherichia coli "selenocysteine insertion sequence" (SECIS) as a multifunctional RNA structure. [Biofactors. 2001]
Review An extended Escherichia coli "selenocysteine insertion sequence" (SECIS) as a multifunctional RNA structure.
Engelberg-Kulka H, Liu Z, Li C, Reches M. Biofactors. 2001; 14(1-4):61-8.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Gene
Gene records that cite the current articles. Citations in Gene are added manually by NCBI or imported from outside public resources.
Nucleotide (Weighted)
Nucleotide records associated with the current articles through the Gene database. These are the related sequences on the Gene record that are added manually by NCBI.
Protein (RefSeq)
NCBI protein Reference Sequences (RefSeqs) that are cited in the current articles, included in the corresponding Gene Reference into Function, or that include the PubMed articles as references.
Protein (Weighted)
Protein records associated with the current articles through related Gene database records. These are the related sequences on the Gene record that are added manually by NCBI.
Protein Clusters
Clusters of related proteins from the Protein Clusters database that cite the current articles. Sources of references in Protein Clusters include the associated Gene and Conserved Domain records as well as NCBI added citations.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Taxonomy via GenBank
Taxonomy records associated with the current articles through taxonomic information on related molecular database records (Nucleotide, Protein, Gene, SNP, Structure).
GEO Profiles
Gene Expression Omnibus (GEO) Profiles of molecular abundance data. The current articles are references on the Gene record associated with the GEO profile.

Recent activity

Clear Turn Off Turn On

Molecular switch in tandem winged-helix motifs of elongation factor SelB.
Molecular switch in tandem winged-helix motifs of elongation factor SelB.
Nucleic Acids Symp Ser (Oxf). 2007 ;(51):377-8.

PubMed
Towards evidence based emergency medicine: best BETs from the Manchester Royal I...
Towards evidence based emergency medicine: best BETs from the Manchester Royal Infirmary. Detection of acute thoracic aortic dissection with D-dimer.
Emerg Med J. 2007 Dec ;24(12):846-7.

PubMed
Several indicators of oxidative stress, immunity, and illness improved in traine...
Several indicators of oxidative stress, immunity, and illness improved in trained men consuming an encapsulated juice powder concentrate for 28 weeks.
J Nutr. 2007 Dec ;137(12):2737-41.

PubMed
Almond consumption reduces oxidative DNA damage and lipid peroxidation in male s...
Almond consumption reduces oxidative DNA damage and lipid peroxidation in male smokers.
J Nutr. 2007 Dec ;137(12):2717-22.

PubMed
Resveratrol potentiates genistein's antiadipogenic and proapoptotic effects in 3...
Resveratrol potentiates genistein's antiadipogenic and proapoptotic effects in 3T3-L1 adipocytes.
J Nutr. 2007 Dec ;137(12):2668-73.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: