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Appl Spectrosc. 2007 Nov;61(11):1233-7.

Raman spectroscopic measurement of relative concentrations in mixtures of oral bacteria.

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  • 1The Institute of Optics, University of Rochester, Rochester, NY 14627, USA.

Abstract

Near-infrared Raman spectroscopy has been used for species identification of pure microbial specimens for more than a decade. More recently, this optical method has been extended to the analysis of specimens containing multiple species. In this report, we demonstrate rapid, reagent-free quantitative analysis of a simplified model of oral plaque containing three oral bacteria species, S. mutans, S. sanguis, and S. gordonii, using near-infrared Raman spectroscopy. Raman spectra were acquired from bacterial mixtures in 200 seconds. A prediction model was calibrated by the partial least squares method and validated by additional samples. On a scale from 0 to 1, relative fractions of each species could be predicted with a root mean square error of 0.07. These results suggest that near-infrared Raman spectroscopy is potentially useful in quantification of microbial mixtures in general and oral plaques in particular.

PMID:
18028703
[PubMed - indexed for MEDLINE]
PMCID:
PMC2475336
Free PMC Article
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