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    Chem Biol. 2007 Nov;14(11):1215-20.

    In vivo resolution of conflicting in vitro results: synthesis of biotin from dethiobiotin does not require pyridoxal phosphate.

    Abdel-Hamid AM, Cronan JE.

    Source

    Department of Microbiology, University of Illinois, Urbana, IL 61801, USA.

    Abstract

    The source of the biotin sulfur atom remains a contested point in studies of biotin synthase (BioB) in vitro. Recent reports that BioB has an intrinsic pyridoxal phosphate (PLP)-dependent cysteine desulfurase activity were tested by depleting Escherichia coli cells of PLP. The BioB-dependent conversion of dethiobiotin to biotin proceeded in these cells irrespective of the presence or absence of PLP.

    PMID:
    18022560
    [PubMed - indexed for MEDLINE]

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