Size-exclusion chromatography based on silica-diol for the analysis of the proinsulin fusion protein

J Chromatogr A. 2007 Dec 28;1176(1-2):157-62. doi: 10.1016/j.chroma.2007.10.090. Epub 2007 Nov 4.

Abstract

Size-exclusion chromatography based on silica-diol sorbent was employed to analyze the recombinant proinsulin fusion protein obtained during the process of refolding and the following ion-exchange purification. The assay was qualified as a control method estimating its accuracy, precision, linearity, limit of detection, limits of quantitation, specificity, and robustness. The results show the reliability for the intended use.

Publication types

  • Validation Study

MeSH terms

  • Chromatography, Gel / methods*
  • Chromatography, High Pressure Liquid / methods*
  • Electrophoresis, Polyacrylamide Gel
  • Proinsulin / analysis*
  • Recombinant Fusion Proteins / analysis*
  • Reproducibility of Results
  • Sensitivity and Specificity

Substances

  • Recombinant Fusion Proteins
  • Proinsulin