J Chromatogr A. 2007 Dec 28;1176(1-2):157-62. Epub 2007 Nov 4.

Size-exclusion chromatography based on silica-diol for the analysis of the proinsulin fusion protein.

Gusarova V, Vorobjeva T, Gusarov D, Lasman V, Bayramashvili D.

Source

Experimental Biotechnological Plant, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of Russian Academy of Sciences, Mikloukho-Maklaya Street, 16/10, Moscow 117997, Russian Federation. gusarovavd@list.ru

Abstract

Size-exclusion chromatography based on silica-diol sorbent was employed to analyze the recombinant proinsulin fusion protein obtained during the process of refolding and the following ion-exchange purification. The assay was qualified as a control method estimating its accuracy, precision, linearity, limit of detection, limits of quantitation, specificity, and robustness. The results show the reliability for the intended use.

PMID:: 18021787; [PubMed - indexed for MEDLINE]

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