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FEBS Lett. 2007 Dec 11;581(29):5601-4. Epub 2007 Nov 20.

Membrane topology of the Drosophila OR83b odorant receptor.

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  • 1Center for Biomembrane Research, Department of Biochemistry and Biophysics, Stockholm University, Svante Arrheniusv 12, SE-106 91, Stockholm, Sweden.


By analogy to mammals, odorant receptors (ORs) in insects, such as Drosophila melanogaster, have long been thought to belong to the G-protein coupled receptor (GPCR) superfamily. However, recent work has cast doubt on this assumption and has tentatively suggested an inverted topology compared to the canonical N(out) - C(in) 7 transmembrane (TM) GPCR topology, at least for some Drosophila ORs. Here, we report a detailed topology mapping of the Drosophila OR83b receptor using engineered glycosylation sites as topology markers. Our results are inconsistent with a classical GPCR topology and show that OR83b has an intracellular N-terminus, an extracellular C-terminus, and 7TM helices.

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