Format

Send to

Choose Destination
See comment in PubMed Commons below
Bioconjug Chem. 2008 Jan;19(1):358-61. Epub 2007 Nov 10.

Selection of reaction additives used in the preparation of monomeric antibody-calicheamicin conjugates.

Author information

  • 1Wyeth Research, 401 N. Middletown Road, Pearl River, New York 10965, USA.

Abstract

The formation of protein aggregates can be a major problem during the preparation of antibody-drug conjugates. Herein is described the methods by which reaction additives were selected, which reduce the tendency of antibodies to aggregate during the attachment of the cytotoxic agent calicheamicin to form an immunoconjugate. Reaction conditions were delineated that produced optimized yields of monomeric conjugates. These conditions were used in the preclinical preparations of gemtuzumab ozogamicin (Mylotarg), the first commercially available chemotherapeutic immunoconjugate.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Write to the Help Desk