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    J Am Chem Soc. 2007 Dec 5;129(48):14858-9. Epub 2007 Nov 9.

    Essential role of an active-site guanine in glmS ribozyme catalysis.

    Klein DJ, Been MD, Ferré-D'Amaré AR.

    Division of Basic Sciences, Fred Hutchinson Cancer Research Center, 1100 Fairview Avenue North, Seattle, Washington 98109, USA.

    The glmS ribozyme is a catalytic riboswitch that is activated for endonucleolytic cleavage by the coenzyme glucosamine-6-phosphate. Using kinetic assays and X-ray crystallography, we identify an active-site mutation of a conserved guanine that abolishes catalysis without perturbing coenzyme binding. Our results provide evidence that coenzyme function requires a specific nucleobase to interact with the nucleophile of the cleavage reaction.

    PMID: 17990888 [PubMed - indexed for MEDLINE]

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