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Proc Natl Acad Sci U S A. 2007 Nov 13;104(46):18085-90. Epub 2007 Nov 6.

PAX3-FOXO1 controls expression of the p57Kip2 cell-cycle regulator through degradation of EGR1.

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  • 1Ludwig Institute for Cancer Research, Biomedical Sciences Graduate Program, University of California at San Diego, 9500 Gilman Drive, La Jolla, CA 92093-0660, USA.

Abstract

The chimeric protein PAX3-FOXO1, resulting from a translocation between chromosomes 2 and 13, is the most common genetic aberration in the alveolar subtype of the human skeletal muscle tumor, rhabdomyosarcoma. To understand how PAX3-FOXO1 contributes to tumor development, we isolated and characterized muscle cells from transgenic mice expressing PAX3-FOXO1 under control of the PAX3 promoter. We demonstrate that these myoblasts are unable to complete myogenic differentiation because of an inability to up-regulate p57Kip2 transcription. This defect is caused by reduced levels of the EGR1 transcriptional activator resulting from a direct, destabilizing interaction with PAX3-FOXO1. Neither PAX3 nor FOXO1 share the ability to regulate p57Kip2 transcription. Thus, the breakage and fusion of the genes encoding these transcription factors creates a unique chimeric protein that controls a key cell-cycle and -differentiation regulator.

PMID:
17986608
[PubMed - indexed for MEDLINE]
PMCID:
PMC2084300
Free PMC Article
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