Biochem Biophys Res Commun. 2008 Jan 11;365(2):285-90. Epub 2007 Nov 5.

Wnt5a-mediated non-canonical Wnt signalling regulates human endothelial cell proliferation and migration.

Cheng CW, Yeh JC, Fan TP, Smith SK, Charnock-Jones DS.

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Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK. cwc28@cam.ac.uk

Abstract

Cell to cell interaction is one of the key processes effecting angiogenesis and endothelial cell function. Wnt signalling is mediated through cell-cell interaction and is involved in many developmental processes and cellular functions. In this study, we investigated the possible function of Wnt5a and the non-canonical Wnt pathway in human endothelial cells. We found that Wnt5a-mediated non-canonical Wnt signalling regulated endothelial cell proliferation. Blocking this pathway using antibody, siRNA or a down-stream inhibitor led to suppression of endothelial cell proliferation, migration, and monolayer wound closure. We also found that the mRNA level of Wnt5a is up-regulated when endothelial cells are treated with a cocktail of inflammatory cytokines. Our findings suggest non-canonical Wnt signalling plays a role in regulating endothelial cell growth and possibly in angiogenesis.

PMID:: 17986384; [PubMed - indexed for MEDLINE]

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