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1: Nat Methods. 2007 Dec;4(12):1011-3. Epub 2007 Nov 4.Click here to read Links

Quantifying small numbers of antibodies with a 'near-universal' protein-DNA chimera.

Burbulis I, Yamaguchi K, Yu R, Resnekov O, Brent R.

Center for Quantitative Genome Function and Molecular Sciences Institute, 2168 Shattuck Avenue, Berkeley, California 94704, USA.

We present general means to greatly increase the sensitivity of antibody-based assays. Augmentation relies on a 'tadpole' protein-DNA chimera whose protein moiety binds most classes of mammalian antibodies but not avian immunoglobulin Y (IgY). We used this tadpole in affinity capture assays followed by real-time PCR to quantify numerous molecules, including prostate-specific antigen (PSA) in human serum, with great sensitivity and accuracy.

PMID: 17982460 [PubMed - indexed for MEDLINE]