Quantitative proteomics of intracellular Porphyromonas gingivalis

Proteomics. 2007 Dec;7(23):4323-37. doi: 10.1002/pmic.200700543.

Abstract

Whole-cell quantitative proteomic analyses were conducted to investigate the change from an extracellular to intracellular lifestyle for Porphyromonas gingivalis, a Gram-negative intracellular pathogen associated with periodontal disease. Global protein abundance data for P. gingivalis strain ATCC 33277 internalized for 18 h within human gingival epithelial cells and controls exposed to gingival cell culture medium were obtained at sufficient coverage to provide strong evidence that these changes are profound. A total of 385 proteins were overexpressed in internalized P. gingivalis relative to controls; 240 proteins were shown to be underexpressed. This represented in total about 28% of the protein encoding ORFs annotated for this organism, and slightly less than half of the proteins that were observed experimentally. Production of several proteases, including the classical virulence factors RgpA, RgpB, and Kgp, was decreased. A separate validation study was carried out in which a 16-fold dilution of the P. gingivalis proteome was compared to the undiluted sample in order to assess the quantitative false negative rate (all ratios truly alternative). Truly null (no change) abundance ratios from technical replicates were used to assess the rate of quantitative false positives over the entire proteome. A global comparison between the direction of abundance change observed and previously published bioinformatic gene pair predictions for P. gingivalis will assist with future studies of P. gingivalis gene regulation and operon prediction.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adhesins, Bacterial / analysis
  • Adhesins, Bacterial / metabolism
  • Bacterial Proteins / analysis
  • Bacterial Proteins / metabolism*
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Databases, Protein
  • Down-Regulation
  • Epithelial Cells / microbiology
  • Gingiva / cytology
  • Humans
  • Intracellular Space / microbiology
  • Peptide Hydrolases / analysis
  • Peptide Hydrolases / metabolism
  • Porphyromonas gingivalis / chemistry
  • Porphyromonas gingivalis / metabolism*
  • Proteome / analysis
  • Proteome / metabolism*
  • Proteomics / methods*
  • Reproducibility of Results
  • Tandem Mass Spectrometry
  • Transcription Factors / analysis
  • Transcription Factors / metabolism
  • Up-Regulation

Substances

  • Adhesins, Bacterial
  • Bacterial Proteins
  • Proteome
  • Transcription Factors
  • Peptide Hydrolases