Howard Hughes Medical Institute, Rosenstiel Center-MS029, Brandeis University, 415 South Street, Waltham, MA 02545-9110, USA.
A low beam intensity, low electron dose imaging method has been developed for single-particle electron cryo-microscopy (cryo-EM). Experiments indicate that the new technique can reduce beam-induced specimen movement and secondary radiolytic effects, such as "bubbling". The improvement in image quality, especially for multiple-exposure data collection, will help single-particle cryo-EM to reach higher resolution.