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Anticancer Res. 2007 Nov-Dec;27(6A):3743-7.

Anti-patched-1 antibodies suppress hedgehog signaling pathway and pancreatic cancer proliferation.

Author information

  • 1Department of Cancer Therapy and Research, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan. mnaka@surg1.med.kyushu-u.ac.jp

Abstract

BACKGROUND:

The hedgehog (Hh) signaling pathway is aberrantly activated in many human carcinomas including pancreatic cancer and regulates tumor cell growth. Overproduction of sonic hedgehog (Shh), a ligand of the Hh signaling pathway, increases the Hh signaling activity through transmitting the signal to patched-1 (Ptch1), the receptor of the Hh signaling pathway.

MATERIALS AND METHODS:

a-Ptch1 antibodies were raised against an oligo-peptide, designed according to the Ptch1 aminoacid sequence. The specificity of a-Ptch1 was examined by immunoblotting and immuno-fluorescence, and biological effects were detected by RT-PCR and cell proliferation assay using two pancreatic cancer cell lines, Panc1 and SUIT-2.

RESULTS:

a-Ptch1 recognized a 160 kDa protein as shown by immunoblotting and cell surface staining of pancreatic cancer cells. Incubation with a-Ptch1 suppressed Hh signaling activity and proliferation of pancreatic cancer cells.

CONCLUSION:

These results provide a new strategy for controling Hh dependent development of pancreatic cancer and other Hh related carcinomas.

PMID:
17970037
[PubMed - indexed for MEDLINE]
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