(A) Success rate distributions of protein production for targets rank-ordered by production feasibility score S p = (P length * P pI * P GRAVY * P tm)1/4. Results of two jackknife tests applied to S p feasibility score are also shown. S ps is a success rate distribution obtained when S p feasibility score was based on the data from four large PSI centers (JCSG, MCSG, NESG, and NYSGXRC) and used to rank-order targets from all other centers (BSGC, BCGI, CESG, ISFI, OPPF, S2F, SECSG, SGPP, SPINE-EU, YSG, TB, and RSGI). For S pl distribution, the targets from the four large PSI centers were rank-ordered using S p feasibility score derived from the data from all other centers. The benchmark result, based on targets processed after our original analysis, is shown as S pb. Because tested sets have different average success rates (from 44%–51%), the normalized plot is shown as an inset. Production probability distribution obtained for OB-Score is shown for comparison (dotted line). (B) Distribution of targets into feasibility classes and observed numbers of successes and failures in protein production.