Section of Eukaryotic Transposable Elements, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Currently, no transposon-based method for the mutagenesis of Schizosaccharomyces pombe exists. We have developed such a system based on the introduction of the hermes transposon from the housefly into S. pombe. This system efficiently disrupts open reading frames and allows the insertion sites to be readily identified.