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Nat Methods. 2007 Nov;4(11):903-5. Epub 2007 Oct 14.

Direct selection of human genomic loci by microarray hybridization.

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  • 1NimbleGen Systems Inc., 1 Science Court, Madison, Wisconsin 53711, USA. talbert@nimblegen.com

Abstract

We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.

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PMID:
17934467
[PubMed - indexed for MEDLINE]
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