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Nat Methods. 2007 Nov;4(11):891-2.
Direct selection of human genomic loci by microarray hybridization.
Albert TJ,
Molla MN,
Muzny DM,
Nazareth L,
Wheeler D,
Song X,
Richmond TA,
Middle CM,
Rodesch MJ,
Packard CJ,
Weinstock GM,
Gibbs RA.
NimbleGen Systems Inc., 1 Science Court, Madison, Wisconsin 53711, USA. talbert@nimblegen.com
We applied high-density microarrays to the enrichment of specific sequences from the human genome for high-throughput sequencing. After capture of 6,726 approximately 500-base 'exon' segments, and of 'locus-specific' regions ranging in size from 200 kb to 5 Mb, followed by sequencing on a 454 Life Sciences FLX sequencer, most sequence reads represented selection targets. These direct selection methods supersede multiplex PCR for the large-scale analysis of genomic regions.
PMID: 17934467 [PubMed - indexed for MEDLINE]