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Nucleic Acids Res. 2008 Jan;36(Database issue):D866-70. Epub 2007 Oct 11.

Many Microbe Microarrays Database: uniformly normalized Affymetrix compendia with structured experimental metadata.

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  • 1Program in Bioinformatics, Boston University, 24 Cummington St. and Department of Biomedical Engineering, Boston University, 44 Cummington St., Boston, Massachusetts, 02215, USA.


Many Microbe Microarrays Database (M3D) is designed to facilitate the analysis and visualization of expression data in compendia compiled from multiple laboratories. M3D contains over a thousand Affymetrix microarrays for Escherichia coli, Saccharomyces cerevisiae and Shewanella oneidensis. The expression data is uniformly normalized to make the data generated by different laboratories and researchers more comparable. To facilitate computational analyses, M3D provides raw data (CEL file) and normalized data downloads of each compendium. In addition, web-based construction, visualization and download of custom datasets are provided to facilitate efficient interrogation of the compendium for more focused analyses. The experimental condition metadata in M3D is human curated with each chemical and growth attribute stored as a structured and computable set of experimental features with consistent naming conventions and units. All versions of the normalized compendia constructed for each species are maintained and accessible in perpetuity to facilitate the future interpretation and comparison of results published on M3D data. M3D is accessible at

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